Sugarcane is cultivated both in tropical and sub-tropical region with the production of large amount of bio mass. Its’ spatial distribution at global level is restricted as per the suitability of various climatic parameters. The soil climate change is now accelerating due to natural, as well as hazardous human activities which disturbing the composition of soil atmosphere, and these changes in soil and atmospheric temperature may affect the sugarcane production in near future. A microbial soil conditioner (Rutoz), containing multiple species of endo mycorrhizae in a potent carrier of humic acid, was utilized in the present study to check the effect of microbial mycorrhiza product (Rutoz) on soil properties, dehydrogenose activity, growth and yield of sugarcane in randomized block design with four replications. The treatments were T1 100% NPK + Rutoz @ 10 kg ha-¹ (basal), T2 100% NK + 75% P + Rutoz @ 10 kg ha-1 (basal), T3 100% NPK + Rutoz @ 10 kg ha-1 (basal) + 10 kg ha at 45 DAP, T4 100% NK + 75% P + Rutoz @ 10 kg ha-¹ (basal) + 10 kg ha-1 at 45 DAP, T5 100%, NPK
and T6 100% NK + 75% P. Pooled data of three years showed significantly improved cane yield (80-84 t/ha) with treatment T3. Change in CCS % was not observed due to any treatments. Maximum B:C ratio (1.88) and increased dehydrogenase activity in soil (228 μg TPF g-¹ ha-1 soil) was also recorded in T3 treatment. The study suggested that soil properties and yield contributing traits can be improved by the use of microbial soil conditioner like Rutoz@ 10 kg/ha as basal dose.